Journal: Pharmacognosy Magazine

Article Title: Bee Pollen and Doxorubicin by Synergistic Effects Inhibit the Proliferation of Breast Tumors in 4T1 Tumor-bearing BALB/c Mice: A Biochemical, Immunohistochemical,and Molecular Approach

doi: 10.1177/09731296231203809

Figure Lengend Snippet: Figure 3. The Mean Mice Serum Levels of LH, FSH (µIU/mL), E2 (pg/mL), and T (ng/dL) in Experimental Groups.

Article Snippet: The levels of E2 (catalog number: KA1907), FSH (catalog number: KA2330), LH (catalog number: NBP2-68054), and T (catalog number: NBP2-42044) hormones were measured in the serum using commercially available Novus ELISA kits based on a colorimetric assay, following the manufacturer’s recommendations and protocol (Ibrahim et al., 2021).

Techniques:

Journal: Cells

Article Title: Activation of MT1/MT2 to Protect Testes and Leydig Cells against Cisplatin-Induced Oxidative Stress through the SIRT1/Nrf2 Signaling Pathway

doi: 10.3390/cells11101690

Figure Lengend Snippet: Effects of platinum chemotherapeutic drugs on the levels of gonadotropin, melatonin, and oxidative stress in the peripheral blood of male gastrointestinal tumor patients, and reducing the expression of MT1/MT2 in the testis. ( A ) Human testicular tissue was stained with H&E (scale bar = 50 μm). ( B ) Images of TUNEL staining in human testicular tissue (scale bar = 50 μm). ( C ) Representative immunofluorescent pictures of MT1 in human testicular tissue; 3β-HSD was tagged with red fluorescence and MT1 was tagged with green fluorescence. The nucleus was labeled with DAPI (scale bar = 20 μm). ( D ) Representative immunofluorescent pictures of MT2 in human testicular tissue; 3β-HSD was tagged with red fluorescence and MT2 was tagged with green fluorescence. The nucleus was labeled with DAPI (scale bar = 20 μm). ( E ) MT1 mRNA expression in human testicular tissue. ( F ) MT2 mRNA expression in human testicular tissue. ( G ) GnRH level in human serum. ( H ) FSH level in human serum. ( I ) LH level in human serum. ( J ) Testosterone level in human serum. ( K ) Melatonin level in human serum. ( L ) Content of MDA in human serum. ( M ) SOD activity in human serum. ( N ) Total antioxidant capacity in human serum. Data are presented as the mean ± SEM. N = 3 cases per group in ( A – F ). N = 30 cases per group in ( G – N ). CT, chemotherapy. * p < 0.05, ** p < 0.01 versus the control group or the tumor group.

Article Snippet: A Mouse MT (melatonin) ELISA Kit (Cat#: E-EL-M0788c), Human MT (melatonin) ELISA Kit (Cat#: E-EL-H2016c), GnRH (gonadotropin-releasing hormone) ELISA Kit (Cat#: E-EL-0071c), T (testosterone) ELISA Kit (Cat#: E-EL-0155c), Human FSH (follicle stimulating hormone) ELISA Kit (Cat#: E-EL-H1143c), and Human LH (luteinizing hormone) ELISA Kit (Cat#: E-EL-H6019) were purchased from Elabscience (Wuhan, China).

Techniques: Expressing, Staining, TUNEL Assay, Fluorescence, Labeling, Activity Assay, Control

Journal: Pharmaceutics

Article Title: A Novel Follitropin Analog Inhibits Follitropin Activity In Vitro

doi: 10.3390/pharmaceutics13030325

Figure Lengend Snippet: In vitro activity of FSH variants. ( A ) Biological activities of FSH variants were determined by measurement of induced cAMP in the media. CHO-dhFr cells stably expressing FSHR were exposed to ascending concentrations of FSH variants (0–500 mU/mL) for 20 h. Each curve is the mean ± SE of at least 5 independent experiments ( n ≥ 5), with 3 replicates for each concentration in each experiment. One-way ANOVA showed a dose-dependent increase in activity following exposure to rhFSH-WT ( p < 0.001). Two-way ANOVA indicated significant differences between FSH variants ( p < 0.001). ( B ) Competitive activity of single-chain FSH β deg - α deg vs rhFSH-WT. FSHR-expressing CHO cells were incubated for 20 h with 10 mU/mL of rhFSH-WT and with ascending concentrations of FSH β deg - α deg (0–500 mU/mL). One-way ANOVA indicated significant effects at 200 and 500 mU/mL ( p < 0.001).

Article Snippet: In order to carry out receptor binding and bioactivity assays, CHO/dhFr - cells were stably transfected with the cDNA of human FSH receptor (FSHR) (ORIGINE, Rockville, MD, USA).

Techniques: In Vitro, Activity Assay, Stable Transfection, Expressing, Concentration Assay, Incubation

Journal: Pharmaceutics

Article Title: A Novel Follitropin Analog Inhibits Follitropin Activity In Vitro

doi: 10.3390/pharmaceutics13030325

Figure Lengend Snippet: The primers used for gene amplification in PCR reactions.

Article Snippet: In order to carry out receptor binding and bioactivity assays, CHO/dhFr - cells were stably transfected with the cDNA of human FSH receptor (FSHR) (ORIGINE, Rockville, MD, USA).

Techniques: Amplification